rat cell line pc12 Search Results


pc12  (CH Instruments)
90
CH Instruments pc12
Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in <t>PC12</t> cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.
Pc12, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pc12/product/CH Instruments
Average 90 stars, based on 1 article reviews
pc12 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
rat neuronal pc12 cell line  (Biopharm GmbH)
90
Biopharm GmbH rat neuronal pc12 cell line
Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in <t>PC12</t> cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.
Rat Neuronal Pc12 Cell Line, supplied by Biopharm GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat neuronal pc12 cell line/product/Biopharm GmbH
Average 90 stars, based on 1 article reviews
rat neuronal pc12 cell line - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pc12, rat pheochromocytoma cell line  (Medim GmbH)
90
Medim GmbH pc12, rat pheochromocytoma cell line
Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in <t>PC12</t> cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.
Pc12, Rat Pheochromocytoma Cell Line, supplied by Medim GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pc12, rat pheochromocytoma cell line/product/Medim GmbH
Average 90 stars, based on 1 article reviews
pc12, rat pheochromocytoma cell line - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in PC12 cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.

Journal: Neural Regeneration Research

Article Title: Srgap2 suppression ameliorates retinal ganglion cell degeneration in mice

doi: 10.4103/1673-5374.369122

Figure Lengend Snippet: Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in PC12 cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.

Article Snippet: PC12, one of the most commonly used cell lines in neuroscience research (You et al., 2022), was purchased from CHI Scientific (Jiangyin, China; RRID: CVCL_0481).

Techniques: Protein-Protein interactions, Inhibition, Expressing, Immunofluorescence, Staining, Cell Culture, Transfection, Activation Assay, Two Tailed Test, Control, Small Interfering RNA